Quantitative reverse transcription PCR is a delicate approach for evaluating transcriptional profiles in numerous experimental datasets. To acquire a dependable quantification of the transcripts stage, knowledge normalization with secure reference genes is required. Steady reference genes are recognized after evaluation of their transcripts profile in each new experiment and species of curiosity.
In Silybum marianum, a broadly cultivated officinal plant, solely few gene expression research exist, and reference genes for RT-qPCR research within the various plant tissues have by no means been investigated earlier than. On this work, the expression stability of 10 candidate reference genes was evaluated in leaves, roots, stems and fruits of S. marianum grown below physiological environmental situation.
The soundness values for every candidate reference gene have been calculated by 4 canonical statistical algorithms GeNorm, NormFinder, Bestkeeper and ΔCt methodology in numerous subsets of samples, then they have been ranked with RefFinder from essentially the most to the least appropriate for normalization. Greatest mixtures of reference genes are lastly proposed for various experimental knowledge units, together with all tissues, vegetative, and reproductive tissues individually.
Three goal genes putatively concerned in vital biosynthetic pathway resulting in key metabolites within the fruits of milk thistle, corresponding to silymarin and fatty acids, have been analyzed with the chosen panels of reference genes, compared to those utilized in earlier papers. To one of the best of our data, that is the primary report on a dependable and systematic identification and validation of the reference genes for RT-qPCR normalization to review gene expression in S. marianum.
Analysis of the Biotoxis qPCR detection ® package for Francisella tularensis detection in scientific and environmental samples
Speedy and dependable detection and identification of Francisella tularensis (a Tier 1 choose agent) are of major curiosity for each medical and organic risk surveillance functions. The Biotoxis qPCR detection® package is a real-time PCR (qPCR) assay designed for the detection of Bacillus anthracis, Yersinia pestis, and F. tularensis in environmental or organic samples. Right here, we evaluated its efficiency for detecting F. tularensis compared to beforehand validated qPCR assays.
The Biotoxis qPCR was constructive for 87/87 F. tularensis subsp. holarctica (kind B) strains, but in addition for F. tularensis subsp. novicida It was unfavourable for F. philomiragia and 24/24 strains belonging to different bacterial species. For 31 tularemia scientific specimens, the Biotoxis qPCR displayed a sensitivity between 90.32% and 96.55%, in comparison with qPCR assessments focusing on the ISFtu2 (ISFtu2-qPCR) or a Sort B-specific DNA sequence (Sort B-qPCR), respectively. All 30 non-tularemia scientific specimens have been Biotoxis qPCR unfavourable. For water samples, the Biotoxis qPCR restrict of detection was 1,000 CFU/l of F. tularensis.
For 57 environmental water samples collected in France, the Biotoxis qPCR was constructive for six/15 samples constructive for ISFtu2-qPCR and 4/Four constructive for Sort B-qPCR.In conclusion, the Biotoxis qPCR detection® package demonstrated good performances for F. tularensis detection in varied organic and environmental samples, though cross-amplification of F. tularensis subsp. novicida have to be thought of. This plate format assay may very well be helpful to check numerous scientific or environmental specimens, particularly within the context of pure or intentional tularemia outbreaks.
The pseudogene drawback and RT-qPCR knowledge normalization; SYMPK: an acceptable reference gene for papillary thyroid carcinoma
In RT-qPCR, accuracy requires a number of ranges of standardization, however outcomes may very well be obfuscated by human errors and technical limitations. Knowledge normalization towards appropriate reference genes is essential, but their noticed expression will be confounded by pseudogenes. Eight reference genes have been chosen based mostly on literature overview and evaluation of papillary thyroid carcinoma (PTC) microarray knowledge. RNA extraction and cDNA synthesis have been adopted by RT-qPCR amplification in triplicate with exon-junction or intron-spanning primers.

A number of statistical analyses have been utilized utilizing Microsoft Excel, NormFinder, and BestKeeper. In regular tissues, the least correlation of variation (CqCV%) and the bottom most fold change (MFC) have been respectively recorded for PYCR1 and SYMPK. In PTC tissues, SYMPK had the bottom CqCV% (5.16%) and MFC (1.17). In keeping with NormFinder, one of the best reference mixture was SYMPK and ACTB (stability worth = 0.209). BestKeeper prompt SYMPK as one of the best reference in each regular (r = 0.969) and PTC tissues (r = 0.958). SYMPK is usually recommended as one of the best reference gene for overcoming the pseudogene drawback in RT-qPCR knowledge normalization, with a stability worth of 0.319.
Subject Identification of Matricaria chamomilla utilizing a Transportable qPCR System
High quality management in botanical merchandise begins with the uncooked materials provide. Historically, botanical identification is carried out by way of morphological evaluation and chemical analytical strategies. Nevertheless, the dearth of availability of botanists, particularly in recent times, coupled with the necessity to improve high quality management to fight the stresses on the availability chain introduced by rising shopper demand and local weather change, necessitates different approaches. The objective of this protocol is to facilitate botanical species identification utilizing a conveyable qPCR system on the sphere or in any setting, the place entry to laboratory tools and experience is restricted.
Goal DNA is amplified utilizing dye-based qPCR, with DNA extracted from botanical reference supplies serving as a constructive management. The goal DNA is recognized by its particular amplification and matching its melting peak towards the constructive management. An in depth description of the steps and parameters, from hands-on subject pattern assortment, to DNA extraction, PCR amplification, adopted by knowledge interpretation, has been included to make sure that readers can replicate this protocol.
Pro QPCR SuperMix Kit |
K5053200 |
Biochain |
200 reactions |
EUR 188 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit |
K5053400 |
Biochain |
400 reactions |
EUR 299 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Green qPCR SuperMix |
20-abx098031 |
Abbexa |
-
EUR 1038.00
-
EUR 439.00
-
EUR 634.00
|
|
- Shipped within 5-10 working days.
|
Probe qPCR SuperMix |
20-abx098040 |
Abbexa |
-
EUR 1038.00
-
EUR 439.00
-
EUR 634.00
|
|
- Shipped within 5-10 working days.
|
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
EUR 1135.00
-
EUR 453.00
-
EUR 662.00
|
|
- Shipped within 5-10 working days.
|
Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
|
|
- Shipped within 5-10 working days.
|
Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
|
|
- Shipped within 5-10 working days.
|
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
|
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- Shipped within 5-10 working days.
|
Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 188 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 299 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
Abbexa |
|
-
100 rxns ×20 ul Systems
-
50 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 408 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 594 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 211 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 320 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
|
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- Shipped within 5-10 working days.
|
Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
|
|
- Shipped within 5-10 working days.
|
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 718 |
- Shipped within 5-10 working days.
|
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 293 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
HS-100-QPCR |
CORNING |
100/pk |
EUR 318 |
Description: Sealing Products; Sealing films - Axygen |
PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
HS-150-QPCR |
CORNING |
100/pk |
EUR 318 |
Description: Sealing Products; Sealing films - Axygen |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 244 |
PCR SuperMix |
20-abx098887 |
Abbexa |
|
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- Shipped within 5-10 working days.
|
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 89 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 141 |
PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
|
- Shipped within 5-10 working days.
|
PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 6174.00
-
EUR 258.00
|
|
- Shipped within 5-10 working days.
|
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
Plus PCR SuperMix |
20-abx098888 |
Abbexa |
|
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- Shipped within 5-10 working days.
|
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
|
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- Shipped within 5-10 working days.
|
Evo? cDNA Supermix |
M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
|
EUR 267 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
|
EUR 441 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
|
EUR 289 |
qPCR Control Template Kit |
G929 |
ABM |
100 rxns |
EUR 148 |
QPCR Positive Control Kit |
M1126-100 |
Biovision |
|
EUR 403 |
Retrovirus qPCR Quantification Kit |
K1472-100 |
Biovision |
100 Rxns |
Ask for price |
Lentivirus qPCR Quantification Kit |
K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-01 |
CORNING |
1/pk |
EUR 80 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 2L, EVA FILM |
91-200-02 |
CORNING |
1/pk |
EUR 88 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
91-200-05 |
CORNING |
1/pk |
EUR 99 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-10 |
CORNING |
1/pk |
EUR 101 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-20 |
CORNING |
1/pk |
EUR 110 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-36 |
CORNING |
1/pk |
EUR 86 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-39 |
CORNING |
1/pk |
EUR 89 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-42 |
CORNING |
1/pk |
EUR 73 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
91-200-43 |
CORNING |
1/pk |
EUR 151 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
91-200-45 |
CORNING |
1/pk |
EUR 126 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
91-200-47 |
CORNING |
1/pk |
EUR 132 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 50L, EVA FILM |
91-200-48 |
CORNING |
1/pk |
EUR 146 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 100L, EVA FILM |
91-200-82 |
CORNING |
1/pk |
EUR 279 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 200L, EVA FILM |
91-200-83 |
CORNING |
1/pk |
EUR 292 |
Description: Flexible Containers; Bioprocess Bags and Parts |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
Methylamp MS-qPCR Fast Kit |
P-1028 |
EpiGentek |
200 Reactions |
EUR 496.6 |
Description: fast delivery possible |
Universal qPCR Mixture |
QPCR1000-UNIV |
BBI Biotech |
4X1.25ml |
EUR 276.2 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA900A-1 |
SBI |
20 profiles |
EUR 850 |
- Category: Long Non-coding RNA Tools
|
COLLECTION CONTAINER 500 ML, EVA FILM |
91-200-41 |
CORNING |
1/pk |
EUR 69 |
Description: Flexible Containers; Bioprocess Bags and Parts |
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
PCR Mycoplasma Detection Kit |
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
|
|
- Shipped within 5-10 working days.
|
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA930A-1 |
SBI |
20 profiles |
EUR 850 |
- Category: Long Non-coding RNA Tools
|
Coronavirus Rapid RT-qPCR Detection Kit |
K1461-100 |
Biovision |
100 Rxns |
EUR 551 |
Adeno-Associated Virus qPCR Quantification Kit |
K1473-100 |
Biovision |
100 Rxns |
EUR 348 |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
Neural Lineage qPCR Profiler |
RA500A-1 |
SBI |
10 reactions |
EUR 999 |
- Category: Lineage Profilers
|
Cancer MicroRNA qPCR Array |
RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
|
Regulatory RNA qPCR Profiler |
RA950A-1 |
SBI |
20 rxn |
EUR 880 |
- Category: Long Non-coding RNA Tools
|
Protein Eva-1 Homolog C (EVA1C) Antibody |
20-abx302696 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
-
100 ug
-
1 mg
-
200 ug
-
20 ug
-
50 ug
|
- Shipped within 5-10 working days.
|
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 376 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 572 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 456 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1453 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 732 |
- Shipped within 5-10 working days.
|
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 648 |
- Shipped within 5-10 working days.
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
|
- Shipped within 5-10 working days.
|
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS750A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS770A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS790A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 153 |
|
COVID-19 RT-qPCR Rapid Detection Kit |
G628 |
ABM |
NULL |
EUR 0 |
One.Step RT-qPCR Probe Kit (2X concentrated) |
105-500 |
GeneOn |
2 x 1,25 ml |
EUR 458 |
One.Step RT-qPCR Probe Kit (2X concentrated) |
105-502 |
GeneOn |
10 x 1,25 ml |
EUR 1719 |
piggyBac qPCR copy number kit, 20 reactions |
PBC100A-1 |
SBI |
20 reactions |
EUR 331 |
- Category: PiggyBac Technology
|
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN320A-KIT |
SBI |
1 Kit |
EUR 4941 |
- Category: PinPoint Integrase Tools
|
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 4941 |
- Category: PinPoint Integrase Tools
|
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
RA910A-1 |
SBI |
20 profiles |
EUR 1108 |
- Category: Long Non-coding RNA Tools
|
HotTaq Probe qPCR Mix (ROX) |
BT11001 |
Bioatlas |
250rxn |
EUR 109 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq Probe qPCR Mix (Capillary) |
BT11003 |
Bioatlas |
250rxn |
EUR 109 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq Probe qPCR Universal Mix |
BT11004 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (ROX) |
BT11101 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (Capillary) |
BT11103 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Universal Mix |
BT11104 |
Bioatlas |
250rxn |
EUR 138 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 263 |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 291 |
BrightGreen 5X qPCR MasterMix-iCycler |
MasterMix-5C |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-ROX |
MasterMix-5P |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-iCycler |
MasterMix-5PC |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-Multiplex |
MasterMix-5PM |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
BrightGreen 5X qPCR MasterMix-ROX |
MasterMix-5R |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
BrightGreen 2X qPCR MasterMix-iCycler |
MasterMix-iC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-iCycler |
MasterMix-iC-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
KiloGreen 2X qPCR MasterMix-iCycler |
MasterMix-KC |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
KiloGreen 2X qPCR MasterMix-ROX |
MasterMix-KR |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
BrightGreen miRNA qPCR MasterMix-iCycler |
MasterMix-mC |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
BrightGreen miRNA qPCR MasterMix-ROX |
MasterMix-mR |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
TaqProbe 2X qPCR MasterMix-ROX |
MasterMix-P |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
TaqProbe 2X qPCR MasterMix-iCycler |
MasterMix-PC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
TaqProbe 2X qPCR MasterMix-Multiplex |
MasterMix-PM |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
MasterMix-R-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE620A-KIT |
SBI |
1 kit |
EUR 2132 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE622A-KIT |
SBI |
1 kit |
EUR 2132 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE624A-KIT |
SBI |
1 kit |
EUR 2132 |
|
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 578 |
- Shipped within 5-10 working days.
|
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 606 |
- Shipped within 5-10 working days.
|
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 759 |
- Shipped within 5-10 working days.
|
Green miRNA Two-Step qRT-PCR SuperMix |
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 885 |
- Shipped within 5-10 working days.
|
igScript One Step RT-qPCR Kit - 100 Reactions |
4214 |
Intact Genomics |
1/EA |
EUR 253 |
igScript One Step RT-qPCR Kit - 500 Reactions |
4218 |
Intact Genomics |
1/EA |
EUR 773 |
One.Step RT-qPCR Kit with EvaGreen (2X concentrated) |
105-520 |
GeneOn |
2 x 1,25 ml |
EUR 458 |
One.Step RT-qPCR Kit with EvaGreen (2X concentrated) |
105-522 |
GeneOn |
10 x 1,25 ml |
EUR 1719 |
vWF Acty. Kit |
ABP-ACT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 428 |
vWF Ant. Kit |
ABP-TOT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 394 |
2x SYBR Green qPCR Master Mix |
B21202 |
Bimake |
5 mL |
EUR 224 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
B21203 |
Bimake |
25 mL |
EUR 856 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
HotTaq Probe qPCR Mix (no ROX) |
BT11002 |
Bioatlas |
250rxn |
EUR 109 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
HotTaq EvaGreen qPCR Mix (no ROX) |
BT11102 |
Bioatlas |
250rxn |
EUR 123 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
Opti-Seal qPCR and Storage Films |
G495 |
ABM |
100 Films/Box |
EUR 202 |
Opti-Seal qPCR 384 Well Plate |
G935 |
ABM |
10 Plates/Box |
EUR 89 |
Opti-Seal qPCR 384 Well Plate |
G936 |
ABM |
100 Plates/Case |
EUR 428 |
Universal miRNA qPCR Primer (10 µM) |
abx298006-200ul |
Abbexa |
200 ul |
EUR 189 |
- Shipped within 5-10 working days.
|
BrightGreen 5X qPCR MasterMix-Low ROX |
MasterMix-5L |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-Low ROX |
MasterMix-5PL |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
TaqProbe 5X qPCR MasterMix-No Dye |
MasterMix-5PS |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
BrightGreen 5X qPCR MasterMix-No Dye |
MasterMix-5S |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
BrightGreen Express 2X qPCR MasterMix-iCycler |
MasterMix-EC |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen Express 2X qPCR MasterMix-ROX |
MasterMix-ER |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
KiloGreen 2X qPCR MasterMix-Low ROX |
MasterMix-KL |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
KiloGreen 2X qPCR MasterMix-No Dye |
MasterMix-KS |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-Low ROX |
MasterMix-LR |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-Low ROX |
MasterMix-LR-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
BrightGreen miRNA qPCR MasterMix-Low ROX |
MasterMix-mL |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
BrightGreen miRNA qPCR MasterMix-No Dye |
MasterMix-mS |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
TaqProbe 2X qPCR MasterMix-Low ROX |
MasterMix-PL |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
TaqProbe 2X qPCR MasterMix-No Dye |
MasterMix-PS |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-No Dye |
MasterMix-S |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
BrightGreen 2X qPCR MasterMix-No Dye |
MasterMix-S-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 376 |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9) |
CAS620A-KIT |
SBI |
1 kit |
EUR 2152 |
|
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression) |
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN300A-KIT |
SBI |
1 Kit |
EUR 2798 |
- Category: PinPoint Integrase Tools
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN400A-KIT |
SBI |
1 Kit |
EUR 2798 |
- Category: PinPoint Integrase Tools
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN410A-KIT |
SBI |
1 Kit |
EUR 4335 |
- Category: PinPoint Integrase Tools
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN412A-KIT |
SBI |
1 Kit |
EUR 4335 |
- Category: PinPoint Integrase Tools
|
PrecisionX Multiplex gRNA Cloning Kit |
CAS9-GRNA-KIT |
SBI |
10 rxn |
EUR 445 |
|
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
20-abx098007 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 606 |
- Shipped within 5-10 working days.
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 662 |
- Shipped within 5-10 working days.
|
The outcomes produced align with conventional laboratory botanical identification strategies. The protocol is simple to carry out and cost-effective, enabling high quality testing on uncooked supplies as near the purpose of origin of the availability chain as potential.