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Society for Research in Hydrocephalus and Spina Bifida
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Randall Ray
November 19, 2020
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Selection and Validation of Reference Genes for RT-qPCR normalization in different tissues of milk thistle (Silybum marianum, Gaert.)

Selection and Validation of Reference Genes for RT-qPCR normalization in different tissues of milk thistle (Silybum marianum, Gaert.)

Quantitative reverse transcription PCR is a delicate approach for evaluating transcriptional profiles in numerous experimental datasets. To acquire a dependable quantification of the transcripts stage, knowledge normalization with secure reference genes is required. Steady reference genes are recognized after evaluation of their transcripts profile in each new experiment and species of curiosity.

In Silybum marianum, a broadly cultivated officinal plant, solely few gene expression research exist, and reference genes for RT-qPCR research within the various plant tissues have by no means been investigated earlier than. On this work, the expression stability of 10 candidate reference genes was evaluated in leaves, roots, stems and fruits of S. marianum grown below physiological environmental situation.

The soundness values for every candidate reference gene have been calculated by 4 canonical statistical algorithms GeNorm, NormFinder, Bestkeeper and ΔCt methodology in numerous subsets of samples, then they have been ranked with RefFinder from essentially the most to the least appropriate for normalization. Greatest mixtures of reference genes are lastly proposed for various experimental knowledge units, together with all tissues, vegetative, and reproductive tissues individually.

Three goal genes putatively concerned in vital biosynthetic pathway resulting in key metabolites within the fruits of milk thistle, corresponding to silymarin and fatty acids, have been analyzed with the chosen panels of reference genes, compared to those utilized in earlier papers. To one of the best of our data, that is the primary report on a dependable and systematic identification and validation of the reference genes for RT-qPCR normalization to review gene expression in S. marianum.

Analysis of the Biotoxis qPCR detection ® package for Francisella tularensis detection in scientific and environmental samples

Speedy and dependable detection and identification of Francisella tularensis (a Tier 1 choose agent) are of major curiosity for each medical and organic risk surveillance functions. The Biotoxis qPCR detection® package is a real-time PCR (qPCR) assay designed for the detection of Bacillus anthracis, Yersinia pestis, and F. tularensis in environmental or organic samples. Right here, we evaluated its efficiency for detecting F. tularensis compared to beforehand validated qPCR assays.

The Biotoxis qPCR was constructive for 87/87 F. tularensis subsp. holarctica (kind B) strains, but in addition for F. tularensis subsp. novicida It was unfavourable for F. philomiragia and 24/24 strains belonging to different bacterial species. For 31 tularemia scientific specimens, the Biotoxis qPCR displayed a sensitivity between 90.32% and 96.55%, in comparison with qPCR assessments focusing on the ISFtu2 (ISFtu2-qPCR) or a Sort B-specific DNA sequence (Sort B-qPCR), respectively. All 30 non-tularemia scientific specimens have been Biotoxis qPCR unfavourable. For water samples, the Biotoxis qPCR restrict of detection was 1,000 CFU/l of F. tularensis.

For 57 environmental water samples collected in France, the Biotoxis qPCR was constructive for six/15 samples constructive for ISFtu2-qPCR and 4/Four constructive for Sort B-qPCR.In conclusion, the Biotoxis qPCR detection® package demonstrated good performances for F. tularensis detection in varied organic and environmental samples, though cross-amplification of F. tularensis subsp. novicida have to be thought of. This plate format assay may very well be helpful to check numerous scientific or environmental specimens, particularly within the context of pure or intentional tularemia outbreaks.

The pseudogene drawback and RT-qPCR knowledge normalization; SYMPK: an acceptable reference gene for papillary thyroid carcinoma

In RT-qPCR, accuracy requires a number of ranges of standardization, however outcomes may very well be obfuscated by human errors and technical limitations. Knowledge normalization towards appropriate reference genes is essential, but their noticed expression will be confounded by pseudogenes. Eight reference genes have been chosen based mostly on literature overview and evaluation of papillary thyroid carcinoma (PTC) microarray knowledge. RNA extraction and cDNA synthesis have been adopted by RT-qPCR amplification in triplicate with exon-junction or intron-spanning primers.

Selection and Validation of Reference Genes for RT-qPCR normalization in different tissues of milk thistle (Silybum marianum, Gaert.)

A number of statistical analyses have been utilized utilizing Microsoft Excel, NormFinder, and BestKeeper. In regular tissues, the least correlation of variation (CqCV%) and the bottom most fold change (MFC) have been respectively recorded for PYCR1 and SYMPK. In PTC tissues, SYMPK had the bottom CqCV% (5.16%) and MFC (1.17). In keeping with NormFinder, one of the best reference mixture was SYMPK and ACTB (stability worth = 0.209). BestKeeper prompt SYMPK as one of the best reference in each regular (r = 0.969) and PTC tissues (r = 0.958). SYMPK is usually recommended as one of the best reference gene for overcoming the pseudogene drawback in RT-qPCR knowledge normalization, with a stability worth of 0.319.

Subject Identification of Matricaria chamomilla utilizing a Transportable qPCR System

High quality management in botanical merchandise begins with the uncooked materials provide. Historically, botanical identification is carried out by way of morphological evaluation and chemical analytical strategies. Nevertheless, the dearth of availability of botanists, particularly in recent times, coupled with the necessity to improve high quality management to fight the stresses on the availability chain introduced by rising shopper demand and local weather change, necessitates different approaches. The objective of this protocol is to facilitate botanical species identification utilizing a conveyable qPCR system on the sphere or in any setting, the place entry to laboratory tools and experience is restricted.

Goal DNA is amplified utilizing dye-based qPCR, with DNA extracted from botanical reference supplies serving as a constructive management. The goal DNA is recognized by its particular amplification and matching its melting peak towards the constructive management. An in depth description of the steps and parameters, from hands-on subject pattern assortment, to DNA extraction, PCR amplification, adopted by knowledge interpretation, has been included to make sure that readers can replicate this protocol.

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Description: Sealing Products; Sealing films - Axygen

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

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Description: Flexible Containers; Bioprocess Bags and Parts

OneScriptPlus cDNA Synthesis SuperMix

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T PCR SuperMix (-dye)

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Methylamp MS-qPCR Fast Kit

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Description: fast delivery possible

Universal qPCR Mixture

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  • Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq

Frit Kit

FRIT-KIT Next Advance 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 SBI 20 profiles
EUR 850
  • Category: Long Non-coding RNA Tools

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Column Packing Kit

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EUR 1035
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PCR Mycoplasma Detection Kit

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PCR SuperMix for PAGE (+dye)

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  • Category: Long Non-coding RNA Tools

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  • Category: Cas9

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Cancer MicroRNA qPCR Array

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  • Category: MicroRNA Tools

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  • Category: Long Non-coding RNA Tools

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20-abx302696 Abbexa
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QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 Biochain 200 reactions
EUR 376
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QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 Biochain 400 reactions
EUR 572
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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  • Category: Cas9

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  • Category: Cas9

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  • Category: Cas9

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  • Category: Cas9

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  • Category: Cas9

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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

miRNA First-Strand cDNA Synthesis SuperMix

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Probe One-Step qRT-PCR SuperMix

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Fly First-Strand cDNA Synthesis SuperMix

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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

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EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT SBI 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT SBI 10 rxn
EUR 1132
  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT SBI 1 Kit
EUR 153
  • Category: Cas9

COVID-19 RT-qPCR Rapid Detection Kit

G628 ABM NULL
EUR 0

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 GeneOn 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 GeneOn 10 x 1,25 ml
EUR 1719

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 SBI 20 reactions
EUR 331
  • Category: PiggyBac Technology

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT SBI 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT SBI 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 SBI 20 profiles
EUR 1108
  • Category: Long Non-coding RNA Tools

HotTaq Probe qPCR Mix (ROX)

BT11001 Bioatlas 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 Bioatlas 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 Bioatlas 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 Bioatlas 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 Bioatlas 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 Bioatlas 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

SYBR Green qPCR Master Mix

HY-K0501 MedChemExpress 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 MedChemExpress 1 mL (100 rxns)
EUR 291

BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-iCycler

MasterMix-5PC ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex

MasterMix-5PM ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL ABM 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC ABM 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR ABM 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC ABM 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR ABM 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex

MasterMix-PM ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL ABM 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

AceQ qPCR Probe Master Mix

Q112-02 Vazyme 500 rxn (20 μl/rxn)
EUR 221

AceQ qPCR Probe Master Mix

Q112-03 Vazyme 2500 rxn (20 μl/rxn)
EUR 646

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT SBI 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT SBI 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT SBI 1 kit
EUR 2132
  • Category: Gene Editing

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems Abbexa 50 rxns × 20 ul Systems
EUR 578
  • Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems Abbexa 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 606
  • Shipped within 5-10 working days.

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems Abbexa 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 759
  • Shipped within 5-10 working days.

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems Abbexa 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 885
  • Shipped within 5-10 working days.

igScript One Step RT-qPCR Kit - 100 Reactions

4214 Intact Genomics 1/EA
EUR 253

igScript One Step RT-qPCR Kit - 500 Reactions

4218 Intact Genomics 1/EA
EUR 773

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)

105-520 GeneOn 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Kit with EvaGreen (2X concentrated)

105-522 GeneOn 10 x 1,25 ml
EUR 1719

vWF Acty. Kit

ABP-ACT-KIT Abpcorp 12 x 8 microwells
EUR 428

vWF Ant. Kit

ABP-TOT-KIT Abpcorp 12 x 8 microwells
EUR 394

2x SYBR Green qPCR Master Mix

B21202 Bimake 5 mL
EUR 224
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix

B21203 Bimake 25 mL
EUR 856
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

HotTaq Probe qPCR Mix (no ROX)

BT11002 Bioatlas 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)

BT11102 Bioatlas 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Opti-Seal qPCR and Storage Films

G495 ABM 100 Films/Box
EUR 202

Opti-Seal qPCR 384 Well Plate

G935 ABM 10 Plates/Box
EUR 89

Opti-Seal qPCR 384 Well Plate

G936 ABM 100 Plates/Case
EUR 428

Universal miRNA qPCR Primer (10 µM)

abx298006-200ul Abbexa 200 ul
EUR 189
  • Shipped within 5-10 working days.

BrightGreen 5X qPCR MasterMix-Low ROX

MasterMix-5L ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-Low ROX

MasterMix-5PL ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-No Dye

MasterMix-5PS ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-No Dye

MasterMix-5S ABM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen Express 2X qPCR MasterMix-iCycler

MasterMix-EC ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen Express 2X qPCR MasterMix-ROX

MasterMix-ER ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-Low ROX

MasterMix-KL ABM 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-No Dye

MasterMix-KS ABM 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX

MasterMix-LR-XL ABM 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL ABM 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-No Dye

MasterMix-mS ABM 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-Low ROX

MasterMix-PL ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-No Dye

MasterMix-PS ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-No Dye

MasterMix-S ABM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-No Dye

MasterMix-S-XL ABM 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

AceQ Universal SYBR qPCR Master Mix

Q511-02 Vazyme 500 rxn (20 μl/rxn)
EUR 221

AceQ Universal SYBR qPCR Master Mix

Q511-03 Vazyme 2500 rxn (20 μl/rxn)
EUR 646

ChamQ Universal SYBR qPCR Master Mix

Q711-02 Vazyme 500 rxn (20 μl/rxn)
EUR 221

ChamQ Universal SYBR qPCR Master Mix

Q711-03 Vazyme 2500 rxn (20 μl/rxn)
EUR 646

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT SBI 1 kit
EUR 2152
  • Category: Cas9
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT SBI 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT SBI 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT SBI 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT SBI 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT SBI 10 rxn
EUR 445
  • Category: Cas9

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007 Abbexa
  • EUR 217.00
  • EUR 356.00
  • 1 ml
  • 5 ml
  • Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200rxns20ulSystems Abbexa 200 rxns × 20 ul Systems
EUR 606
  • Shipped within 5-10 working days.

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems Abbexa 200 rxns × 20 ul Systems
EUR 662
  • Shipped within 5-10 working days.

 

The outcomes produced align with conventional laboratory botanical identification strategies. The protocol is simple to carry out and cost-effective, enabling high quality testing on uncooked supplies as near the purpose of origin of the availability chain as potential.

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