Selection and Validation of Reference Genes for RT-qPCR normalization in different tissues of milk thistle (Silybum marianum, Gaert.)
Quantitative reverse transcription PCR is a delicate approach for evaluating transcriptional profiles in numerous experimental datasets. To acquire a dependable quantification of the transcripts stage, knowledge normalization with secure reference genes is required. Steady reference genes are recognized after evaluation of their transcripts profile in each new experiment and species of curiosity.
In Silybum marianum, a broadly cultivated officinal plant, solely few gene expression research exist, and reference genes for RT-qPCR research within the various plant tissues have by no means been investigated earlier than. On this work, the expression stability of 10 candidate reference genes was evaluated in leaves, roots, stems and fruits of S. marianum grown below physiological environmental situation.
The soundness values for every candidate reference gene have been calculated by 4 canonical statistical algorithms GeNorm, NormFinder, Bestkeeper and ΔCt methodology in numerous subsets of samples, then they have been ranked with RefFinder from essentially the most to the least appropriate for normalization. Greatest mixtures of reference genes are lastly proposed for various experimental knowledge units, together with all tissues, vegetative, and reproductive tissues individually.
Three goal genes putatively concerned in vital biosynthetic pathway resulting in key metabolites within the fruits of milk thistle, corresponding to silymarin and fatty acids, have been analyzed with the chosen panels of reference genes, compared to those utilized in earlier papers. To one of the best of our data, that is the primary report on a dependable and systematic identification and validation of the reference genes for RT-qPCR normalization to review gene expression in S. marianum.
Analysis of the Biotoxis qPCR detection ® package for Francisella tularensis detection in scientific and environmental samples
Speedy and dependable detection and identification of Francisella tularensis (a Tier 1 choose agent) are of major curiosity for each medical and organic risk surveillance functions. The Biotoxis qPCR detection® package is a real-time PCR (qPCR) assay designed for the detection of Bacillus anthracis, Yersinia pestis, and F. tularensis in environmental or organic samples. Right here, we evaluated its efficiency for detecting F. tularensis compared to beforehand validated qPCR assays.
The Biotoxis qPCR was constructive for 87/87 F. tularensis subsp. holarctica (kind B) strains, but in addition for F. tularensis subsp. novicida It was unfavourable for F. philomiragia and 24/24 strains belonging to different bacterial species. For 31 tularemia scientific specimens, the Biotoxis qPCR displayed a sensitivity between 90.32% and 96.55%, in comparison with qPCR assessments focusing on the ISFtu2 (ISFtu2-qPCR) or a Sort B-specific DNA sequence (Sort B-qPCR), respectively. All 30 non-tularemia scientific specimens have been Biotoxis qPCR unfavourable. For water samples, the Biotoxis qPCR restrict of detection was 1,000 CFU/l of F. tularensis.
For 57 environmental water samples collected in France, the Biotoxis qPCR was constructive for six/15 samples constructive for ISFtu2-qPCR and 4/Four constructive for Sort B-qPCR.In conclusion, the Biotoxis qPCR detection® package demonstrated good performances for F. tularensis detection in varied organic and environmental samples, though cross-amplification of F. tularensis subsp. novicida have to be thought of. This plate format assay may very well be helpful to check numerous scientific or environmental specimens, particularly within the context of pure or intentional tularemia outbreaks.
The pseudogene drawback and RT-qPCR knowledge normalization; SYMPK: an acceptable reference gene for papillary thyroid carcinoma
In RT-qPCR, accuracy requires a number of ranges of standardization, however outcomes may very well be obfuscated by human errors and technical limitations. Knowledge normalization towards appropriate reference genes is essential, but their noticed expression will be confounded by pseudogenes. Eight reference genes have been chosen based mostly on literature overview and evaluation of papillary thyroid carcinoma (PTC) microarray knowledge. RNA extraction and cDNA synthesis have been adopted by RT-qPCR amplification in triplicate with exon-junction or intron-spanning primers.
A number of statistical analyses have been utilized utilizing Microsoft Excel, NormFinder, and BestKeeper. In regular tissues, the least correlation of variation (CqCV%) and the bottom most fold change (MFC) have been respectively recorded for PYCR1 and SYMPK. In PTC tissues, SYMPK had the bottom CqCV% (5.16%) and MFC (1.17). In keeping with NormFinder, one of the best reference mixture was SYMPK and ACTB (stability worth = 0.209). BestKeeper prompt SYMPK as one of the best reference in each regular (r = 0.969) and PTC tissues (r = 0.958). SYMPK is usually recommended as one of the best reference gene for overcoming the pseudogene drawback in RT-qPCR knowledge normalization, with a stability worth of 0.319.
Subject Identification of Matricaria chamomilla utilizing a Transportable qPCR System
High quality management in botanical merchandise begins with the uncooked materials provide. Historically, botanical identification is carried out by way of morphological evaluation and chemical analytical strategies. Nevertheless, the dearth of availability of botanists, particularly in recent times, coupled with the necessity to improve high quality management to fight the stresses on the availability chain introduced by rising shopper demand and local weather change, necessitates different approaches. The objective of this protocol is to facilitate botanical species identification utilizing a conveyable qPCR system on the sphere or in any setting, the place entry to laboratory tools and experience is restricted.
Goal DNA is amplified utilizing dye-based qPCR, with DNA extracted from botanical reference supplies serving as a constructive management. The goal DNA is recognized by its particular amplification and matching its melting peak towards the constructive management. An in depth description of the steps and parameters, from hands-on subject pattern assortment, to DNA extraction, PCR amplification, adopted by knowledge interpretation, has been included to make sure that readers can replicate this protocol.
Probe qPCR SuperMix |
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20-abx098040 | Abbexa |
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Green qPCR SuperMix |
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abx098031-100l | Abbexa | 100 µl | EUR 675 |
Green qPCR SuperMix |
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abx098031-200l | Abbexa | 200 µl | EUR 912.5 |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056200 | Biochain | 200 reactions | EUR 180 |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056400 | Biochain | 400 reactions | EUR 326 |
Green qPCR SuperMix UDG |
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20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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20-abx098034 | Abbexa |
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Green qPCR SuperMix UDG |
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abx098032-100l | Abbexa | 100 µl | EUR 687.5 |
Green qPCR SuperMix UDG |
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abx098032-200l | Abbexa | 200 µl | EUR 912.5 |
Top Green qPCR SuperMix |
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abx098033-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix |
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abx098033-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix |
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abx098034-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix |
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abx098034-200l | Abbexa | 200 µl | EUR 437.5 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058200 | Biochain | 200 reactions | EUR 211 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058400 | Biochain | 400 reactions | EUR 354 |
Library Quantification qPCR SuperMix |
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20-abx098896 | Abbexa |
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Library Quantification qPCR SuperMix |
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abx098896-100l | Abbexa | 100 µl | EUR 700 |
Library Quantification qPCR SuperMix |
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abx098896-200l | Abbexa | 200 µl | EUR 962.5 |
EnTurbo™ probe qPCR SuperMix |
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EQ017 | ELK Biotech | 5mL | EUR 240 |
Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 | Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
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abx098890-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
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abx098890-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
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abx098891-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
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abx098891-200l | Abbexa | 200 µl | EUR 437.5 |
EnTurbo™ SYBR Color qPCR SuperMix |
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EQ036 | ELK Biotech | 5mL | EUR 340 |
cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems | Abbexa |
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HyperScript RT SuperMix for qPCR |
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K1074-100 | ApexBio | 100 rxn (20 uL/rxn) | EUR 280 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
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K1074-50 | ApexBio | 50 rxn (20 uL/rxn) | EUR 150 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
cDNA Synthesis SuperMix for qPCR |
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abx098019-100l | Abbexa | 100 µl | EUR 825 |
cDNA Synthesis SuperMix for qPCR |
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abx098019-1ml | Abbexa | 1 ml | EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
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abx098019-200l | Abbexa | 200 µl | EUR 1000 |
HiScript II Q RT SuperMix for qPCR |
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R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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EQ035 | ELK Biotech | 5mL | EUR 340 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-100l | Abbexa | 100 µl | EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-200l | Abbexa | 200 µl | Ask for price |
HiScript II Q Select RT SuperMix for qPCR |
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R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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R423-01 | Vazyme | 100rxns(20µl/rxn) | EUR 326 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 217.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-100l | Abbexa | 100 µl | EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-1ml | Abbexa | 1 ml | Ask for price |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-200l | Abbexa | 200 µl | Ask for price |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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EQ033 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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EQ034 | ELK Biotech | 5mL | EUR 340 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
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K1174-100 | ApexBio | 100 rxns | EUR 304 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
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K1174-50 | ApexBio | 50 rxns | EUR 176 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
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R223-01 | Vazyme | 100rxns(20µl/rxn) | EUR 159.5 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054200 | Biochain | 200 reactions | EUR 468 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054400 | Biochain | 400 reactions | EUR 711 |
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
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R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 159.5 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054002 | Biochain | 200 rxn | EUR 468 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054004 | Biochain | 400 rxn | EUR 711 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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R333-01 | Vazyme | 100 rxns (21 μl/rxn) | EUR 217.5 |
PCR SuperMix |
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20-abx098887 | Abbexa |
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2X PCR SuperMix |
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MB200-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
PCR SuperMix (-dye) |
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20-abx098002 | Abbexa |
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PCR SuperMix (+dye) |
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20-abx098003 | Abbexa |
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PCR SuperMix (-dye) |
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abx098002-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (-dye) |
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abx098002-200l | Abbexa | 200 µl | EUR 337.5 |
PCR SuperMix (+dye) |
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abx098003-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (+dye) |
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abx098003-200l | Abbexa | 200 µl | EUR 337.5 |
Plus PCR SuperMix |
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20-abx098888 | Abbexa |
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Evo? cDNA Supermix |
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M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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M1168-25 | Biovision | each | EUR 320.4 |
Plus PCR SuperMix |
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abx098888-100l | Abbexa | 100 µl | EUR 300 |
Plus PCR SuperMix |
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abx098888-200l | Abbexa | 200 µl | EUR 612.5 |
Novo? cDNA Supermix |
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M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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M1169-25 | Biovision | each | EUR 346.8 |
Probe Direct SuperMix |
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abx461057-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe Direct SuperMix |
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abx461057-596tests | Abbexa | 5 × 96 tests | EUR 2800 |
Probe Direct SuperMix |
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abx461057-96tests | Abbexa | 96 tests | EUR 812.5 |
T PCR SuperMix (-dye) |
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20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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20-abx098006 | Abbexa |
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T PCR SuperMix (-dye) |
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abx098005-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (-dye) |
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abx098005-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (+dye) |
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abx098006-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (+dye) |
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abx098006-200l | Abbexa | 200 µl | EUR 462.5 |
cDNA Synthesis SuperMix |
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20-abx09801420ulSystems | Abbexa |
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cDNA Synthesis SuperMix |
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abx098014-100l | Abbexa | 100 µl | EUR 725 |
cDNA Synthesis SuperMix |
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abx098014-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix |
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abx098014-200l | Abbexa | 200 µl | EUR 812.5 |
AmpHS 2X PCR SuperMix |
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MB203-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055200 | Biochain | 200 reactions | EUR 427 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055400 | Biochain | 400 reactions | EUR 682 |
EasyPfu PCR SuperMix (-dye) |
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20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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20-abx098010 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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abx098010-100l | Abbexa | 100 µl | EUR 300 |
FastPfu PCR SuperMix (-dye) |
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abx098010-200l | Abbexa | 200 µl | EUR 575 |
Library Amplification SuperMix |
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20-abx098894 | Abbexa |
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Library Amplification SuperMix |
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abx098894-100l | Abbexa | 100 µl | EUR 737.5 |
Library Amplification SuperMix |
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abx098894-200l | Abbexa | 200 µl | EUR 1137.5 |
OneScriptcDNA Synthesis SuperMix |
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G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
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20-abx098004 | Abbexa |
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PCR SuperMix for PAGE (+dye) |
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abx098004-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
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abx098004-200l | Abbexa | 200 µl | EUR 337.5 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055002 | Biochain | 200 rxn | EUR 426 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055004 | Biochain | 400 rxn | EUR 682 |
OneScriptPlus cDNA Synthesis SuperMix |
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G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
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MBA01-0100 | Bio-Helix | 100 rxns (2.5ml) | EUR 16 |
EnTurbo™ SYBR Green PCR SuperMix |
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EQ001 | ELK Biotech | 5mL | EUR 340 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
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MB204-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 13 |
Green Two-Step qRT-PCR SuperMix |
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abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 777.6 |
Green One-Step qRT-PCR SuperMix |
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20-abx09803820ulSystems | Abbexa |
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Probe One-Step qRT-PCR SuperMix |
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20-abx09804220ulSystems | Abbexa |
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Green Two-Step qRT-PCR SuperMix |
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abx098035-100l | Abbexa | 100 µl | EUR 925 |
Green Two-Step qRT-PCR SuperMix |
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abx098035-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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abx098035-200l | Abbexa | 200 µl | Ask for price |
Green One-Step qRT-PCR SuperMix |
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abx098038-100l | Abbexa | 100 µl | EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
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abx098038-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix |
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abx098038-200l | Abbexa | 200 µl | EUR 1050 |
Probe One-Step qRT-PCR SuperMix |
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abx098042-100l | Abbexa | 100 µl | EUR 850 |
Probe One-Step qRT-PCR SuperMix |
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abx098042-1ml | Abbexa | 1 ml | Ask for price |
Probe One-Step qRT-PCR SuperMix |
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abx098042-200l | Abbexa | 200 µl | EUR 1400 |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 727.2 |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 910.8 |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-100l | Abbexa | 100 µl | EUR 887.5 |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-200l | Abbexa | 200 µl | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-100l | Abbexa | 100 µl | EUR 1087.5 |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-200l | Abbexa | 200 µl | Ask for price |
AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X) |
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MB202-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
All-in-One cDNA Synthesis SuperMix |
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B24403 | Bimake | 200 ractions | EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
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B24408 | Bimake | 1000 ractions | EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
OmniPCR HS Supermix w Fluorescent dye |
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MBA03-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OmniPCR FAST Supermix w Fluorescent dye |
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MBA02-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OmniPCR Long Supermix w Fluorescent dye |
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MBA04-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 727.2 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 794.4 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 1028.4 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-100l | Abbexa | 100 µl | EUR 875 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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abx098027-200l | Abbexa | 200 µl | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-100l | Abbexa | 100 µl | EUR 950 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098028-200l | Abbexa | 200 µl | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-100l | Abbexa | 100 µl | EUR 1212.5 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-200l | Abbexa | 200 µl | Ask for price |
Fly First-Strand cDNA Synthesis SuperMix |
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20-abx09885820ulSystems | Abbexa |
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Fly First-Strand cDNA Synthesis SuperMix |
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abx098858-100l | Abbexa | 100 µl | EUR 850 |
Fly First-Strand cDNA Synthesis SuperMix |
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abx098858-1ml | Abbexa | 1 ml | Ask for price |
Fly First-Strand cDNA Synthesis SuperMix |
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abx098858-200l | Abbexa | 200 µl | EUR 1037.5 |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems | Abbexa | 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) | EUR 1062 |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-100l | Abbexa | 100 µl | EUR 1212.5 |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-1ml | Abbexa | 1 ml | Ask for price |
Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-200l | Abbexa | 200 µl | Ask for price |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-20rxns20ulSystems | Abbexa | 20 rxns × 20 ul Systems | EUR 878.4 |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-100l | Abbexa | 100 µl | EUR 950 |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-1ml | Abbexa | 1 ml | Ask for price |
miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-200l | Abbexa | 200 µl | Ask for price |
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
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20-abx098007 | Abbexa |
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Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 961.2 |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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20-abx09803920ulSystems | Abbexa |
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Probe One-Step qRT-PCR SuperMix (GC Rich) |
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20-abx09804120ulSystems | Abbexa |
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High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
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abx098007-100l | Abbexa | 100 µl | EUR 262.5 |
High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
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abx098007-200l | Abbexa | 200 µl | EUR 475 |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-100l | Abbexa | 100 µl | EUR 1125 |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-200l | Abbexa | 200 µl | Ask for price |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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abx098039-100l | Abbexa | 100 µl | EUR 450 |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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abx098039-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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abx098039-200l | Abbexa | 200 µl | EUR 1000 |
Probe One-Step qRT-PCR SuperMix (GC Rich) |
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abx098041-100l | Abbexa | 100 µl | EUR 737.5 |
Probe One-Step qRT-PCR SuperMix (GC Rich) |
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abx098041-1ml | Abbexa | 1 ml | Ask for price |
Probe One-Step qRT-PCR SuperMix (GC Rich) |
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abx098041-200l | Abbexa | 200 µl | EUR 1050 |
First-Strand cDNA Synthesis SuperMix for PCR |
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abx098018-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 693.6 |
First-Strand cDNA Synthesis SuperMix for PCR |
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abx098018-100l | Abbexa | 100 µl | EUR 850 |
First-Strand cDNA Synthesis SuperMix for PCR |
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abx098018-1ml | Abbexa | 1 ml | Ask for price |
First-Strand cDNA Synthesis SuperMix for PCR |
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abx098018-200l | Abbexa | 200 µl | Ask for price |
HyperScript First-Strand cDNA Synthesis SuperMix |
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K1073-100 | ApexBio | 100 rxn (20 uL/rxn) | EUR 282 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix |
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K1073-50 | ApexBio | 50 rxn (20 uL/rxn) | EUR 150 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
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20-abx09885920ulSystems | Abbexa |
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One-Step gDNA Removal and cDNA Synthesis SuperMix |
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abx098859-100l | Abbexa | 100 µl | EUR 1062.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
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abx098859-1ml | Abbexa | 1 ml | Ask for price |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
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abx098859-200l | Abbexa | 200 µl | EUR 1425 |
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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EQ014 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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EQ013 | ELK Biotech | 5mL | EUR 340 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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20-abx09801620ulSystems | Abbexa |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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20-abx09802120ulSystems | Abbexa |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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abx098016-100l | Abbexa | 100 µl | EUR 775 |
The outcomes produced align with conventional laboratory botanical identification strategies. The protocol is simple to carry out and cost-effective, enabling high quality testing on uncooked supplies as near the purpose of origin of the availability chain as potential.