Tuberculosis (TB) is the deadliest of infectious ailments. TB prognosis, based mostly on sputum microscopy, tradition, and nucleic acid amplification checks (NAATs) to determine its fundamental causative agent, Mycobacterium tuberculosis (MTB), stays difficult.
The present accessible NAATs, endorsed by World Well being Group (WHO), can differentiate MTB from some MTB complicated (MTBC) members. Utilizing bioinformatics, we recognized a single nucleotide polymorphism (SNP) in lprM (Rv1970) gene that differentiate MTB from different MTBC members. A ahead mismatch amplification mutation assay (MAMA) primer was designed for the focused mutation and was utilized in a semi-nested melt-MAMA qPCR (lprM-MAMA).
Utilizing the optimized protocol, lprM-MAMA was constructive with all MTB reference and scientific strains, and unfavorable with different MTBC members, non-tuberculous mycobacteria (NTM) and different non-mycobacterial (NM) reference strains. The restrict of detection (LOD) of lprM-MAMA was 76.29 fg. Xpert® MTB/RIF (Xpert)-positive sputum samples had been additionally constructive by lprM-MAMA, aside from samples categorized as having “very low” bacterial load by Xpert.
Xpert-negative sputum samples had been additionally unfavorable by lprM-MAMA. In conclusion, lprM-MAMA demonstrated to be a useful gizmo for particular MTB prognosis. Additional analysis with larger variety of reference strains, together with NTM and NM; and sputum samples are required to find out its potential for scientific software.
Evaluating the prevalence and threat elements for macrolide resistance in Mycoplasma genitalium utilizing a newly developed qPCR assay
Mycoplasma genitalium (MG) is a sexually transmitted bacterium during which macrolide resistance is quickly rising, limiting therapy choices. We validated a brand new assay to detect the presence of macrolide resistance-associated mutations in MG (MG-MRAM). In 2018, symptomatic and asymptomatic purchasers visiting sexually transmitted infections (STI) clinics in Amsterdam or The Hague had been examined for MG utilizing transcription mediated amplification (TMA) assays.
The sensitivity to detect MG of the newly developed MG-MRAM qPCR was in comparison with the MgPa qPCR, each in relation to the TMA assay. For the sensitivity and specificity to detect related mutations the MG-MRAM qPCR was in comparison with 23SrRNA sequencing evaluation. The qPCR was subsequently used to find out the presence of MG-MRAM at totally different anatomical areas and to determine threat elements for MG-MRAM. MG-positive purchasers (402) offering 493 MG-positive samples had been included.
In complete 309/493 (62.7%) samples from 291 (72.4%) purchasers had been efficiently typed with the MG-MRAM qPCR. The MG-MRAM qPCR had a sensitivity of 98.6% (95%CI 91.1%-99.9%) and specificity of 94.1% (95%CI 78.9%-99.0%) to detect MG-MRAM in comparison with sequencing evaluation. An infection with MG-MRAM was detected in 193/291 (66.3%) purchasers: in 129/178 (72.5%) males and 64/113 (56.6%) girls (p = 0.005).
Prevalence of MG-MRAM was considerably larger in males, purchasers with a better schooling, HIV-positive purchasers and purchasers with >10 sexual companions within the earlier six months, however in multivariable evaluation no issue was considerably related to MG-MRAM presence. Since MG-MRAM prevalence was very excessive, testing for MG-MRAM is crucial if therapy for MG is taken into account, and could be carried out with this delicate and particular qPCR take a look at in routine diagnostics.
An Exonuclease V-qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Traces and Tissues
Integration of the human papillomavirus (HPV) genome into host cell chromosomes has been noticed in a majority of HPV-positive cervical cancers and a subset of oral HPV-associated cancers. HPV integration additionally happens in long-term cell tradition. Screening for HPV integration could be labor intensive and yield outcomes which can be troublesome to interpret. Right here we describe an assay based mostly on exonuclease V (ExoV/RecBCD) and quantitative polymerase chain response (qPCR) to find out if samples from cell traces and tissues comprise episomal or built-in HPV.
This assay could be utilized to display screen different small DNA viruses with episomal/linear genome configurations of their viral lifecycle and has the potential for use in scientific settings to outline viral genomic conformations related to illness.
Analytical Sensitivity and Specificity of Two RT-qPCR Protocols for SARS-CoV-2 Detection Carried out in an Automated Workflow
WHO declared the novel coronavirus (COVID-19) outbreak a worldwide pandemic on 11 March 2020. The institution of standardized RT-qPCR protocols for respiratory secretions testing, in addition to sharing of specimens, knowledge, and knowledge turned essential. Right here, we examine the analytical efficiency of two interim RT-qPCR protocols (Charité and Facilities for Illness Management (CDC)) for the qualitative detection of SARS-CoV-2 executed in a completely automated platform.
Analytical specificity, PCR amplification effectivity, analytical sensitivity (restrict of detection), and cross-reactivity had been evaluated utilizing contrived samples. The on-going accuracy was evaluated by retrospective evaluation of our take a look at outcomes database (actual scientific samples). N1, E, and a modified model of RdRP assays offered enough analytical specificity, amplification effectivity, and analytical sensitivity utilizing contrived samples. The three assays had been utilized to all people who requested the SARS-CoV-2 molecular take a look at assay in our laboratory and it was noticed that N1 gave extra constructive outcomes than E, and E gave extra constructive outcomes than RdRP (modified).
) Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 |
Abbexa |
-
EUR 829.00
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EUR 217.00
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EUR 1135.00
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EUR 411.00
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- Shipped within 5-10 working days.
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) Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 |
Abbexa |
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EUR 968.00
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EUR 230.00
-
EUR 1330.00
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EUR 467.00
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- Shipped within 5-10 working days.
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 Probe qPCR SuperMix |
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20-abx098040 |
Abbexa |
-
EUR 1038.00
-
EUR 439.00
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EUR 634.00
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- Shipped within 5-10 working days.
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 Library Quantification qPCR SuperMix |
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20-abx098896 |
Abbexa |
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- Shipped within 5-10 working days.
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 Eva QPCR SuperMix Kit |
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K5052200 |
Biochain |
200 reactions |
EUR 211 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Eva QPCR SuperMix Kit |
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K5052400 |
Biochain |
400 reactions |
EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Pro QPCR SuperMix Kit |
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K5053200 |
Biochain |
200 reactions |
EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit |
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K5053400 |
Biochain |
400 reactions |
EUR 299 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems |
Abbexa |
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100 rxns ×20 ul Systems
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50 rxns × 20 ul Systems
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- Shipped within 5-10 working days.
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 Power Eva QPCR SuperMix Kit |
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K5057200 |
Biochain |
200 reactions |
EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Power Eva QPCR SuperMix Kit |
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K5057400 |
Biochain |
400 reactions |
EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
) OPTIONAL GREEN FILTER (572NM) |
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GDBL-GREEN |
CORNING |
1/pk |
EUR 1052 |
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Description: Lab Equipment; Axygen Branded EQ |
 Pro QPCR SuperMix Kit - ROX premixed |
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K5056200 |
Biochain |
200 reactions |
EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056400 |
Biochain |
400 reactions |
EUR 299 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 SYBR Green qPCR Master Mix |
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HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 263 |
 Green Two-Step qRT-PCR SuperMix |
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abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 648 |
- Shipped within 5-10 working days.
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 Green One-Step qRT-PCR SuperMix |
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20-abx09803820ulSystems |
Abbexa |
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-
100 rxns × 20 ul Systems
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400 rxns × 20 ul Systems
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- Shipped within 5-10 working days.
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) cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 718 |
- Shipped within 5-10 working days.
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 Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058200 |
Biochain |
200 reactions |
EUR 211 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058400 |
Biochain |
400 reactions |
EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 HiScript II Q RT SuperMix for qPCR |
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R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
 2x SYBR Green qPCR Master Mix |
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B21202 |
Bimake |
5 mL |
EUR 224 |
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Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
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B21203 |
Bimake |
25 mL |
EUR 856 |
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Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
 Green miRNA Two-Step qRT-PCR SuperMix |
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abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 885 |
- Shipped within 5-10 working days.
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 HiScript II Q Select RT SuperMix for qPCR |
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R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
) SYBR Green qPCR Master Mix (High ROX) |
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HY-K0521 |
MedChemExpress |
1 mL (100 rxns) |
EUR 113 |
) SYBR Green qPCR Master Mix (Low ROX) |
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HY-K0522 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 257 |
) SYBR Green qPCR Master Mix (No ROX) |
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HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 257 |
 AceQ qPCR SYBR® Green Master Mix |
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Q111-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 221 |
AceQ qPCR SYBR® Green Master Mix |
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Q111-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 646 |
) Green-2-Go qPCR Mastermix (500X20ul Rxn) |
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QPCR004-S |
BBI Biotech |
4X1.25ml |
EUR 252.71 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
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) Green Two-Step qRT-PCR SuperMix (GC Rich) |
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abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 801 |
- Shipped within 5-10 working days.
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) Green One-Step qRT-PCR SuperMix (GC Rich) |
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20-abx09803920ulSystems |
Abbexa |
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100 rxns × 20 ul Systems
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400 rxns × 20 ul Systems
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- Shipped within 5-10 working days.
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) HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 293 |
 PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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HS-100-QPCR |
CORNING |
100/pk |
EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
 PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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HS-150-QPCR |
CORNING |
100/pk |
EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
) 2x SYBR Green qPCR Master Mix (High ROX) |
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B21402 |
Bimake |
5 ml |
EUR 224 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (High ROX) |
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B21403 |
Bimake |
25 ml |
EUR 856 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) 2x SYBR Green qPCR Master Mix (Low ROX) |
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B21702 |
Bimake |
5 ml |
EUR 224 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
2x SYBR Green qPCR Master Mix (Low ROX) |
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B21703 |
Bimake |
25 ml |
EUR 856 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
) Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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QPCR001-R |
BBI Biotech |
4X1.25ml |
EUR 252.71 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
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) Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
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QPCR003-iC |
BBI Biotech |
4X1.25ml |
EUR 252.71 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
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) HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 244 |
 ig SYBR Green qPCR 2X Master Mix - 500 Reactions |
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3356 |
Intact Genomics |
1/EA |
EUR 288 |
) Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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QPCR002-L |
BBI Biotech |
4X1.25ml |
EUR 252.71 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
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) Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
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QPCR006-NODYE |
BBI Biotech |
4X1.25ml |
EUR 271.85 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
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) Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
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QPCR005-NODYE |
BBI Biotech |
4X1.25ml |
EUR 252.71 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
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) Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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W2601-1 |
101Bio |
NULL |
EUR 0 |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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W2601-5 |
101Bio |
NULL |
EUR 0 |
 PCR SuperMix |
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20-abx098887 |
Abbexa |
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- Shipped within 5-10 working days.
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 20 profiles) LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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RA910A-1 |
SBI |
20 profiles |
EUR 1108 |
- Category: Long Non-coding RNA Tools
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 OneScriptcDNA Synthesis SuperMix |
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G451 |
ABM |
25 x 20 ul reactions |
EUR 89 |
OneScriptcDNA Synthesis SuperMix |
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G452 |
ABM |
100 x 20 ul reactions |
EUR 141 |
) PCR SuperMix (-dye) |
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20-abx098002 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
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- Shipped within 5-10 working days.
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) PCR SuperMix (+dye) |
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20-abx098003 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 6174.00
-
EUR 258.00
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- Shipped within 5-10 working days.
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 cDNA Synthesis SuperMix |
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20-abx09801420ulSystems |
Abbexa |
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-
100 rxns × 20 ul Systems
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50 rxns × 20 ul Systems
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- Shipped within 5-10 working days.
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 Plus PCR SuperMix |
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20-abx098888 |
Abbexa |
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- Shipped within 5-10 working days.
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 Library Amplification SuperMix |
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20-abx098894 |
Abbexa |
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- Shipped within 5-10 working days.
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 Evo? cDNA Supermix |
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M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
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M1168-25 |
Biovision |
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EUR 267 |
 Novo? cDNA Supermix |
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M1169-100 |
Biovision |
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EUR 441 |
Novo? cDNA Supermix |
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M1169-25 |
Biovision |
|
EUR 289 |
 OneScriptPlus cDNA Synthesis SuperMix |
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G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
) T PCR SuperMix (-dye) |
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20-abx098005 |
Abbexa |
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- Shipped within 5-10 working days.
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) T PCR SuperMix (+dye) |
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20-abx098006 |
Abbexa |
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- Shipped within 5-10 working days.
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) EasyPfu PCR SuperMix (-dye) |
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20-abx098009 |
Abbexa |
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- Shipped within 5-10 working days.
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) FastPfu PCR SuperMix (-dye) |
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20-abx098010 |
Abbexa |
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- Shipped within 5-10 working days.
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 Universal qPCR Mixture |
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QPCR1000-UNIV |
BBI Biotech |
4X1.25ml |
EUR 276.2 |
- Product category: PCR Related/PCR Premix (qPCR)/qPCR Premix + Taq
|
 Leucomalachite green |
|
AG105 |
Unibiotest |
1 mg |
EUR 523 |
Leucomalachite green |
|
AT105 |
Unibiotest |
1mg |
EUR 1368 |
 Brilliant green |
|
BB0242 |
BBI Biotech |
25g |
EUR 56.09 |
- Product category: Biochemicals/Indicators/Stains/Biological Stains
|
 Bromocresol green |
|
20-abx186394 |
Abbexa |
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- Shipped within 1-2 weeks.
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 MitoView Green |
|
70054 |
Biotium |
20X50UG |
EUR 226 |
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Description: Minimum order quantity: 1 unit of 20X50UG |
) PCR SuperMix for PAGE (+dye) |
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20-abx098004 |
Abbexa |
-
EUR 411.00
-
EUR 189.00
-
EUR 258.00
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- Shipped within 5-10 working days.
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 qPCR Control Template Kit |
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G929 |
ABM |
100 rxns |
EUR 148 |
 QPCR Positive Control Kit |
|
M1126-100 |
Biovision |
|
EUR 403 |
 Neural Lineage qPCR Profiler |
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RA500A-1 |
SBI |
10 reactions |
EUR 999 |
- Category: Lineage Profilers
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 Cancer MicroRNA qPCR Array |
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RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
|
|
 Regulatory RNA qPCR Profiler |
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RA950A-1 |
SBI |
20 rxn |
EUR 880 |
- Category: Long Non-coding RNA Tools
|
 Retrovirus qPCR Quantification Kit |
|
K1472-100 |
Biovision |
100 Rxns |
Ask for price |
 Lentivirus qPCR Quantification Kit |
|
K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
 All-in-One cDNA Synthesis SuperMix |
|
B24403 |
Bimake |
200 ractions |
EUR 456 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
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B24408 |
Bimake |
1000 ractions |
EUR 1453 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
 miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 732 |
- Shipped within 5-10 working days.
|
 Probe One-Step qRT-PCR SuperMix |
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20-abx09804220ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
400 rxns × 20 ul Systems
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- Shipped within 5-10 working days.
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 Fly First-Strand cDNA Synthesis SuperMix |
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20-abx09885820ulSystems |
Abbexa |
|
-
100 rxns × 20 ul Systems
-
50 rxns × 20 ul Systems
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- Shipped within 5-10 working days.
|
 Leuco- Malachiet green |
|
AG131 |
Unibiotest |
1 mg |
EUR 523 |
) Indocyanine green(Cardiogreen) |
|
E1KS1924 |
EnoGene |
50 mg |
EUR 247 |
 Fast Green FCF |
|
FB0452 |
BBI Biotech |
5g |
EUR 55.22 |
- Product category: Biochemicals/Indicators/Stains/Peptide/Protein Related
|
 Safe-Green™ |
|
G108-G |
ABM |
1.0 ml |
EUR 71 |
Fast Green FCF |
|
abx082188-5g |
Abbexa |
5 g |
EUR 203 |
- Shipped within 5-10 working days.
|
 C.I.Solvent Green 3 |
|
abx185369-25g |
Abbexa |
25 g |
EUR 509 |
- Shipped within 1-2 weeks.
|
 Malachite green oxalate |
|
abx180007-25g |
Abbexa |
25 g |
EUR 272 |
- Shipped within 1-2 weeks.
|
 Leucomalachite green-HRP |
|
80-1192 |
Fitzgerald |
500 ul |
EUR 137 |
|
Description: Leucomalachite green Conjugate for use in immunoassays |
 BactoViewâ„¢ Live Green |
|
40102 |
Biotium |
100uL |
EUR 320 |
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Description: Minimum order quantity: 1 unit of 100uL |
 Malachite green Antibody |
|
48363-100ul |
SAB |
100ul |
EUR 333 |
Malachite green Antibody |
|
48363-50ul |
SAB |
50ul |
EUR 239 |
 PhosLite™ Green |
|
11630 |
AAT Bioquest |
1 mg |
EUR 219 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12171501
|
The RdRP and E had been faraway from the take a look at and its last model, based mostly on N1 assay solely, was utilized to 30,699 Brazilian people (from 19 February 2020 to eight Could 2020). The aggregated take a look at outcomes accessible within the database had been additionally offered.
