The identification of laboratory markers which predict the end result of COVID-19 sufferers is a good concern. Actual-time reverse transcriptase-polymerase chain response (RT-PCR) has been used to substantiate the scientific analysis. The purpose of this examine is to guage laboratory parameters of COVID-19 sufferers in addition to to guage the RT-PCR crossing level (Cp) worth and correlate blood check abnormalities and the Cp worth with sufferers survival. 200 thirty sufferers with optimistic RT-PCR of SARS-CoV-2 had been included within the examine. Molecular analysis of SARS-CoV-2 was carried out by RT-PCR.
Scientific data, biochemical parameters and Cp values had been collected in an anonymized database and variables had been analyzed with SPSS v25.0 (IBM Company, Armonk, NY, USA). No-survivors had been considerably older than survivors. The next prevalence of cardiovascular comorbidities in sufferers who died than in those that survived was discovered. Statistically important variations had been obtained evaluating RT-PCR Cp values for the E-gene of sufferers who died and those that survived, being decrease these of sufferers who died. No-survivors had considerably larger ranges of CRP. E-gene Cp values <=28, which correlate with a excessive variety of copies of SARS-CoV-2, in addition to a number of demographical and biochemical parameters could possibly be helpful markers of loss of life threat in these sufferers.
Commercially obtainable reverse transcription-polymerase chain response (RT-PCR) kits are getting used as an essential instrument to diagnose SARS-CoV-2 an infection in scientific laboratories worldwide. Nonetheless, some kits lack adequate scientific analysis as a result of want for emergency use attributable to the present COVID-19 pandemic. Right here we discovered {that a} novel insertion/deletion mutation within the nucleocapsid (N) gene of SARS-CoV-2 samples is a reason behind unfavourable outcomes for the N gene in a broadly used assay that obtained emergency use authorization (EUA) from US FDA and Conformite Europeenne-in vitro diagnostics (CE-IVD) from EU.
Though SARS-CoV-2 is recognized optimistic by different goal probes within the assay, our findings present an proof of the genetic variability and speedy evolution of SARS-CoV-2 in addition to a reference in designing industrial RT-PCR assays. The evaluation and prediction of workflow included the variety of RT-PCR per week from the start of the pandemic, with a complete of 31971 determinations. The planning part was developed primarily based on the completely different situations beforehand predicted.
RT–PCR Screening Checks for SARS-CoV-2 with Saliva Samples in Asymptomatic Folks: Technique to Preserve Social and Financial Actions whereas Decreasing the Threat of Spreading the Virus
The yr 2020 will probably be remembered for the coronavirus illness 2019 (COVID-19) pandemic, which continues to have an effect on the entire world. Early and correct identification of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is prime to fight the illness. Among the many present diagnostic checks, real-time reverse transcriptase-polymerase chain response (RT-qPCR) is probably the most dependable and incessantly used technique. Herein, we talk about the interpretation of RT-qPCR outcomes relative to viral infectivity. Though nasopharyngeal swab samples are sometimes used for RT-qPCR testing, they require assortment by educated medical workers. Saliva samples are rising as an affordable and environment friendly various for large-scale screening.
Pooled-sample testing of saliva has been utilized for mass screening of SARS-CoV-2 an infection. Present insurance policies advocate isolating folks with borderline cycle threshold (Ct) values (35<Ct <40), regardless of these Ct values indicating minimal an infection threat. We suggest the brand new idea of a “social cut-off” Ct worth and threat stratification primarily based on the correlation of Ct with infectivity. We additionally describe the expertise of RT-qPCR screening of saliva samples at our establishment. You will need to implement a scientific strategy to reduce viral transmission whereas permitting financial and social actions to proceed.
Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) an infection has quickly unfold worldwide from the start of 2020. Quantitative reverse transcription-PCR (RT-qPCR) is, to at the present time, the popular methodology for viral RNA detection, even when not with out issues. To beat a few of the limitations nonetheless present for the detection and quantification of nucleic acids in numerous functions, the usage of one-step reverse transcription-droplet digital PCR (RT-ddPCR) has been established. The aim of this examine was, then, to guage the efficacy of ddPCR for the detection of SARS-CoV-2 RNA in nasopharyngeal swabs, optimizing the detection of low-viral load-burdened samples.
Detection of feline norovirus utilizing industrial real-time RT–PCR equipment for the analysis of human norovirus an infection
Feline noroviruses (FNoVs) are potential scientific pathogens in cats. To carry out an epidemiological examine of FNoV an infection, it’s essential to develop a easy and efficient technique for virus detection. We investigated whether or not a industrial human NoV quantitative RT-PCR equipment for the detection of human NoVs utilized in medical observe could be utilized for FNoV detection. This equipment was able to detecting the FNoV gene whatever the genogroup (GIV and GVI) in experimental and area samples. Based mostly on the above findings, it’s potential to detect FNoVs utilizing human NoV checks.
cDNA Synthesis SuperMix for qPCR |
abx098019-200l |
Abbexa |
200 µl |
EUR 1000 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
|
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-100l |
Abbexa |
100 µl |
EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-1ml |
Abbexa |
1 ml |
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cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-200l |
Abbexa |
200 µl |
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cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
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cDNA Synthesis SuperMix |
abx098014-100l |
Abbexa |
100 µl |
EUR 725 |
cDNA Synthesis SuperMix |
abx098014-1ml |
Abbexa |
1 ml |
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cDNA Synthesis SuperMix |
abx098014-200l |
Abbexa |
200 µl |
EUR 812.5 |
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 693.6 |
|
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-100l |
Abbexa |
100 µl |
EUR 850 |
First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-1ml |
Abbexa |
1 ml |
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First-Strand cDNA Synthesis SuperMix for PCR |
abx098018-200l |
Abbexa |
200 µl |
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OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
20-abx09801620ulSystems |
Abbexa |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
20-abx09802120ulSystems |
Abbexa |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
abx098016-100l |
Abbexa |
100 µl |
EUR 775 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
abx098016-1ml |
Abbexa |
1 ml |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
abx098016-200l |
Abbexa |
200 µl |
EUR 912.5 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
abx098021-100l |
Abbexa |
100 µl |
EUR 962.5 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
abx098021-1ml |
Abbexa |
1 ml |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
abx098021-200l |
Abbexa |
200 µl |
EUR 1237.5 |
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 878.4 |
|
All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-100l |
Abbexa |
100 µl |
EUR 1050 |
All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-1ml |
Abbexa |
1 ml |
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All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
abx098023-200l |
Abbexa |
200 µl |
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Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
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Fly First-Strand cDNA Synthesis SuperMix |
abx098858-100l |
Abbexa |
100 µl |
EUR 850 |
Fly First-Strand cDNA Synthesis SuperMix |
abx098858-1ml |
Abbexa |
1 ml |
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Fly First-Strand cDNA Synthesis SuperMix |
abx098858-200l |
Abbexa |
200 µl |
EUR 1037.5 |
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 878.4 |
|
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-100l |
Abbexa |
100 µl |
EUR 950 |
miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-1ml |
Abbexa |
1 ml |
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miRNA First-Strand cDNA Synthesis SuperMix |
abx098020-200l |
Abbexa |
200 µl |
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HyperScript First-Strand cDNA Synthesis SuperMix |
K1073-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 282 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix |
K1073-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 150 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
20-abx09885920ulSystems |
Abbexa |
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One-Step gDNA Removal and cDNA Synthesis SuperMix |
abx098859-100l |
Abbexa |
100 µl |
EUR 1062.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix |
abx098859-1ml |
Abbexa |
1 ml |
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One-Step gDNA Removal and cDNA Synthesis SuperMix |
abx098859-200l |
Abbexa |
200 µl |
EUR 1425 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
20-abx09802220ulSystems |
Abbexa |
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One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
abx098022-100l |
Abbexa |
100 µl |
EUR 1012.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
abx098022-1ml |
Abbexa |
1 ml |
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One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
abx098022-200l |
Abbexa |
200 µl |
EUR 1325 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
20-abx09801520ulSystems |
Abbexa |
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One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
20-abx09801720ulSystems |
Abbexa |
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One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
abx098015-100l |
Abbexa |
100 µl |
EUR 750 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
abx098015-1ml |
Abbexa |
1 ml |
EUR 1737.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
abx098015-200l |
Abbexa |
200 µl |
EUR 862.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
abx098017-100l |
Abbexa |
100 µl |
EUR 812.5 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
abx098017-1ml |
Abbexa |
1 ml |
EUR 2225 |
One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
abx098017-200l |
Abbexa |
200 µl |
EUR 962.5 |
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper) |
K1173-100 |
ApexBio |
100 rxns |
EUR 304 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript First-Strand cDNA Synthesis SuperMix (with gDNA wiper) |
K1173-50 |
ApexBio |
50 rxns |
EUR 176 |
Description: Reverse transcription reaction premixed solution for efficient synthesis of first-strand cDNA. |
HyperScript RT SuperMix for qPCR |
K1074-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 280 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
K1074-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 150 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
EntiLink™ 1st Strand cDNA Synthesis Super Mix |
EQ031 |
ELK Biotech |
100 rxn |
EUR 399 |
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 326 |
LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA900A-1 |
SBI |
20 profiles |
EUR 1020 |
|
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 217.5 |
Green qPCR SuperMix |
20-abx098031 |
Abbexa |
-
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Probe qPCR SuperMix |
20-abx098040 |
Abbexa |
-
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|
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Green qPCR SuperMix |
abx098031-100l |
Abbexa |
100 µl |
EUR 675 |
Green qPCR SuperMix |
abx098031-200l |
Abbexa |
200 µl |
EUR 912.5 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-100 |
ApexBio |
100 rxns |
EUR 304 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-50 |
ApexBio |
50 rxns |
EUR 176 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
R223-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 159.5 |
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
RA930A-1 |
SBI |
20 profiles |
EUR 1020 |
|
Eva QPCR SuperMix Kit |
K5052200 |
Biochain |
200 reactions |
EUR 211 |
Eva QPCR SuperMix Kit |
K5052400 |
Biochain |
400 reactions |
EUR 354 |
Pro QPCR SuperMix Kit |
K5053200 |
Biochain |
200 reactions |
EUR 180 |
Pro QPCR SuperMix Kit |
K5053400 |
Biochain |
400 reactions |
EUR 326 |
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
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Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
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Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
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|
|
|
Green qPCR SuperMix UDG |
abx098032-100l |
Abbexa |
100 µl |
EUR 687.5 |
Green qPCR SuperMix UDG |
abx098032-200l |
Abbexa |
200 µl |
EUR 912.5 |
Top Green qPCR SuperMix |
abx098033-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix |
abx098033-200l |
Abbexa |
200 µl |
EUR 487.5 |
Tip Green qPCR SuperMix |
abx098034-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix |
abx098034-200l |
Abbexa |
200 µl |
EUR 437.5 |
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 159.5 |
Fast Eva QPCR SuperMix Kit |
K5052002 |
Biochain |
200 rxn |
EUR 211 |
Power Eva QPCR SuperMix Kit |
K5057200 |
Biochain |
200 reactions |
EUR 180 |
Power Eva QPCR SuperMix Kit |
K5057400 |
Biochain |
400 reactions |
EUR 354 |
EnTurbo™ probe qPCR SuperMix |
EQ017 |
ELK Biotech |
5mL |
EUR 240 |
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
-
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Library Quantification qPCR SuperMix |
abx098896-100l |
Abbexa |
100 µl |
EUR 700 |
Library Quantification qPCR SuperMix |
abx098896-200l |
Abbexa |
200 µl |
EUR 962.5 |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
-
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Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
-
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Top Green qPCR SuperMix (+Dye II) |
abx098890-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
abx098890-200l |
Abbexa |
200 µl |
EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
abx098891-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
abx098891-200l |
Abbexa |
200 µl |
EUR 437.5 |
LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
RA910A-1 |
SBI |
20 profiles |
EUR 1329.6 |
|
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 217.5 |
Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 180 |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 326 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036 |
ELK Biotech |
5mL |
EUR 340 |
cDNA Synthesis Kit |
abx460012-1096tests |
Abbexa |
10 × 96 tests |
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cDNA Synthesis Kit |
abx460012-596tests |
Abbexa |
5 × 96 tests |
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cDNA Synthesis Kit |
abx460012-96tests |
Abbexa |
96 tests |
EUR 262.5 |
cDNA Synthesis Kit |
abx461055-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
cDNA Synthesis Kit |
abx461055-596tests |
Abbexa |
5 × 96 tests |
EUR 1637.5 |
cDNA Synthesis Kit |
abx461055-96tests |
Abbexa |
96 tests |
EUR 450 |
cDNA Synthesis Kit |
abx461073-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
cDNA Synthesis Kit |
abx461073-596tests |
Abbexa |
5 × 96 tests |
EUR 1037.5 |
cDNA Synthesis Kit |
abx461073-96tests |
Abbexa |
96 tests |
EUR 525 |
Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 211 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 354 |
EVery cDNA Synthesis Kit |
EVery200B-1 |
SBI |
20 preps |
EUR 307 |
Tetro cDNA Synthesis Kit |
BIO-65042 |
Bioline |
30 Reactions |
Ask for price |
Tetro cDNA Synthesis Kit |
BIO-65043 |
Bioline |
100 Reactions |
Ask for price |
circRNA cDNA Synthesis Kit |
G627 |
ABM |
25 rxn (20 ul/rxn) |
EUR 370.8 |
SensiFAST cDNA Synthesis Kit |
BIO-65053 |
Bioline |
50 Reactions |
Ask for price |
SensiFAST cDNA Synthesis Kit |
BIO-65053/S |
Bioline |
Sample |
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SensiFAST cDNA Synthesis Kit |
BIO-65054 |
Bioline |
250 Reactions |
Ask for price |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035 |
ELK Biotech |
5mL |
EUR 340 |
OneScriptPlus cDNA Synthesis Kit |
G235 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
Viva cDNA Synthesis Kit, 50app |
cDSK01-050 |
Vivantis |
each |
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Viva cDNA Synthesis Kit, 100app |
cDSK01-100 |
Vivantis |
each |
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RScript™ cDNA Synthesis Kit |
RK001-0050 |
Bio-Helix |
50 rxns |
EUR 60 |
OneScript Plus cDNA Synthesis Kit |
E36G236 |
EnoGene |
100 rxn |
EUR 207.14 |
OneScript Plus cDNA Synthesis Kit |
G236 |
ABM |
100 x 20 ul reactions |
EUR 195 |
Description: OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. |
amfiRivet cDNA Synthesis 2X Buffer |
R5102-050 |
GenDepot |
500ul |
EUR 160.8 |
amfiRivet cDNA Synthesis 2X Buffer |
R5102-100 |
GenDepot |
2x500ul |
EUR 230.4 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034 |
ELK Biotech |
5mL |
EUR 340 |
amfiRivert cDNA Synthesis Master Mix |
R5101-050 |
GenDepot |
50 rxns |
EUR 498 |
amfiRivert cDNA Synthesis Master Mix |
R5101-100 |
GenDepot |
2X50 rxns |
EUR 1016.4 |
amfiRivert cDNA Synthesis Master Mix |
R5101-200 |
GenDepot |
4X50 rxns |
EUR 1453.2 |
EntiLink™1st Strand cDNA SynthesisSuperMix(for qPCRgDNA Eraser plus) |
EQ037 |
ELK Biotech |
20µL x 100 rxns |
EUR 409 |
EpiNext Hi-Fi cDNA Synthesis Kit |
P-9004 |
EpiGentek |
-
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|
OneScript Hot RT cDNA Synthesis Kit |
E36G594 |
EnoGene |
100 rxn |
EUR 271.43 |
OneScript Hot RT cDNA Synthesis Kit |
G594 |
ABM |
100 rxn |
EUR 295 |
Description: OneScript® Hot cDNA Synthesis Kit is the first kit in the world that allows for efficient androbust cDNA synthesis at 60ºC. All other cDNA synthesis kits on the market are optimizedfor the reaction to be performed at 42-55ºC, which is sufficient for most RNA template, butfails on more difficult templates. abm is the only company in the world to have a reversetranscriptase engineered to offer superior cDNA synthesis performance with even the mostchallenging RNA samples due to its incredible thermostability at 60-72ºC. The enzyme is amutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that canreverse transcribe low abundance or degraded RNA, and has significantly better resistanceto contaminating inhibitors such as reagents used during RNA extraction and contaminantsfrom biological samples. High processivity and sensitivity allow for rapid cDNA synthesis offull-length cDNA fragments in a fraction of the time of leading competitors.This kit provides a comprehensive set of reagents necessary to generate high quality cDNAand offers the most flexibility in respect to priming methods and reaction optimization. Bothrandom primers and oligo(dT) are included for a choice of general priming strategies andas alternatives to gene-specific primers. |
Total-Transcriptome cDNA Synthesis Kit |
G904 |
ABM |
25 reactions |
EUR 268.8 |
Total-Transcriptome cDNA Synthesis Kit |
G905 |
ABM |
100 reactions |
EUR 652.8 |
RScript™ cDNA Synthesis Kit (Premix) |
RK002-0050 |
Bio-Helix |
50 rxns |
EUR 60 |
Optimax First Strand cDNA Synthesis Kit |
K4201100 |
Biochain |
100 rnx |
EUR 466 |
amfiRivert II cDNA Synthesis Master Mix |
R5500-050 |
GenDepot |
50 rxns |
EUR 448.8 |
amfiRivert II cDNA Synthesis Master Mix |
R5500-100 |
GenDepot |
2x50rxns |
EUR 692.4 |
amfiRivert II cDNA Synthesis Master Mix |
R5500-500 |
GenDepot |
10x50rxns |
EUR 1410 |
HiFiScript 1st Strand cDNA Synthesis Kit |
W2569-10 |
101Bio |
10 rxn |
EUR 139 |
HiFiScript 1st Strand cDNA Synthesis Kit |
W2569-30 |
101Bio |
30 rxn |
EUR 399 |
HiFiScript 1st Strand cDNA Synthesis Kit |
W2569-100 |
101Bio |
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AzuraQuant cDNA Synthesis Kit - 25 Reactions |
AZ-1995 |
Real Time Primers |
25 Reactions |
EUR 183.6 |
AzuraQuant cDNA Synthesis Kit - 100 Reactions |
AZ-1996 |
Real Time Primers |
100 Reactions |
EUR 446.4 |
amfiRivert cDNA Synthesis Prime Master Mix |
R5610-050 |
GenDepot |
50 rxns |
EUR 494.4 |
The connection between FNoV an infection and gastroenteritis in cats could also be clarified by making use of these strategies to an epidemiological survey of FNoVs. In response to the predictions obtained, an automatic customized resolution was chosen, primarily based on the usage of the OT-2 open-source liquid-handling robots (Opentrons), to design a reproducible workflow that achieved a manufacturing capability of 5640 samples/day, with a time of response of 4 hours per process. The evaluation and prediction of workflow, together with the usage of the robotic platforms OT-2, offered a sturdy construction to take care of the excessive demand of determinations that this pandemic requires.