Identification of a Mycobacterium tuberculosis-specific gene marker for diagnosis of tuberculosis using semi-nested melt-MAMA qPCR

Identification of a Mycobacterium tuberculosis-specific gene marker for diagnosis of tuberculosis using semi-nested melt-MAMA qPCR

Tuberculosis (TB) is the deadliest of infectious ailments. TB prognosis, based mostly on sputum microscopy, tradition, and nucleic acid amplification checks (NAATs) to determine its fundamental causative agent, Mycobacterium tuberculosis (MTB), stays difficult. The present accessible NAATs, endorsed by World Well being Group (WHO), can differentiate MTB from some MTB complicated (MTBC) members. Utilizing bioinformatics, […]

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A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision

A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision

Recombinant adeno-associated virus (rAAV) is without doubt one of the principal vectors utilized in gene remedy. An correct genome titer is just not solely crucial for medical dosing, but additionally a prerequisite for a lot of analytical assays for AAV product characterization. AAV genome titer is historically decided by qPCR; nonetheless, assay precision is just […]

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